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KMID : 0377220010260010016
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Medical Journal of Chosun Univercity
2001 Volume.26 No. 1 p.16 ~ p.22
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Confocal Microscopic Observation of c-Kit- and NK1R-immunoreactive Cellular Network in Guinea Pig Intestine
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Chang In-Youb
Kim Jong-Joong
Moon Jeong-Seok
Kim Hyun-Kon
Park Chan-Guk
Jun Jae-Yeoul
Cheon Kyu-Bae
Cho Cheol-Hee
You Ho-Jin
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Abstract
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Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement.
Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope.
Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY).
Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.
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KEYWORD
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c-Kit, Interstitial cells of Cajal, Intestine, Gastro-intestinal motility, guinea pig
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